Journal: bioRxiv
Article Title: Glial cell and perineuronal net interactions in the dorsal striatum of aged mice
doi: 10.64898/2026.03.25.714174
Figure Lengend Snippet: B. Quantification of GFAP + cell counts for the total striatum. Data was normalized to area. C. Quantification of GFAP + intensity per cell. D. Quantification of GFAP + cell bodv area in the striatum. E. Quantification of average endpoints per GFAP + cell in the striatum. F. Sholl analysis of the branch profile (ramification) of GFAP + cells in the striatum. G. Percentage ofPNNs associated with a GFAP + cells. H. Quantification of WTA intensity per GFAP + cell. I. Quantification of distance of GFAP + cells to soma of PNN-ensheathed neurons. J. Linear regression analysis of GFAP intensity versus WFA + intensity per cell. R 2 values of corresponding linear regressions label on graph. Data represented as mean ± SEM, data was tested for normality prior to statistical analysis. Two-tailed t-tests or Welch’s t-test used when appropriate. Sholl analysis: Two-Way ANOVA with Tukey. Regression analysis: t-test comparison of slopes, p-values: * <0.05, ** <0.01. N = 4-5 mice/group, 3-5 sections/mouse, 13-41 cells/mouse.
Article Snippet: Coronal 30-μm hemisections containing the dorsal striatum were processed in two independent staining/imaging cohorts: ( ) microglia were labeled with Iba1 (rabbit; FUJIFILM Wako, #P3088) in combination with the lysosomal/phagocytic marker CD68 (rat; Bio-Rad, #MCA1957) and perineuronal nets (PNNs) were visualized using fluorescein-conjugated Wisteria floribunda agglutinin (WFA; Vector Laboratories, #FL-1351); ( ) astrocytes were labeled with GFAP (rabbit; Cell Signaling Technology, #12389) together with WFA to visualize PNNs.
Techniques: Two Tailed Test, Comparison